Transfection reagents freeze-thaw 10 times

From ELN
Revision as of 16:31, 22 November 2011 by Luyingying (talk | contribs) (Created page with "Make transfection reagent (TR) to freeze-thaw 10 times. 3 ul TR + 50ul DMEM without serum---->Mix A 1 ug DNA(pCMV-EGFP) + 50ul DMEM without serum---->Mix B Mix A + Mix B...")
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to navigation Jump to search

Make transfection reagent (TR) to freeze-thaw 10 times.

3 ul TR + 50ul DMEM without serum---->Mix A

1 ug DNA(pCMV-EGFP) + 50ul DMEM without serum---->Mix B

Mix A + Mix B, incubate 20min at room temperature

Add 100 ul mixture to transfect 293T cells in 12 well-plate.


48 hours after transfection, picture taken on 11-4-2011

Picture was taken on 11-7-2011.


Retrieved from "https://eln.excellgen.com/index.php?title=Transfection_reagents_freeze-thaw_10_times&oldid=2506"