Transfection reagents freeze-thaw 10 times
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Make transfection reagent (TR) to freeze-thaw 10 times.
3 ul TR + 50ul DMEM without serum---->Mix A
1 ug DNA(pCMV-EGFP) + 50ul DMEM without serum---->Mix B
Mix A + Mix B, incubate 20min at room temperature
Add 100 ul mixture to transfect 293T cells in 12 well-plate.
48 hours after transfection, picture taken on 11-4-2011
Picture was taken on 11-7-2011.