374-pAd5.CMV-Dest-His

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Cloning of 374-pAd5.CMV-Dest-His

Purpose: to clone pAd5.CMV-Dest-His by gene synthesis

Date: 2011-11-9----

Notebook:Lu yingying

Page:

Clone ID:374

Experimenters: lu yingying
Results


PCR gateway cassette using 446-pVP16 vector as template

ad-GW-F,ad-GW-His-R


cut with Kpn1 + Xho1
ligate to Kpn1 +Xho1 digested vector280


Date: 2011-11-15----

Notebook:Lu yingying

Page:

Clone ID:374

Experimenters: lu yingying
Results


PCR gateway cassette using 446-pVP16 vector as template

ad-GW-F,ad-GW-His-R


cut with Kpn1 + Xho1
ligate to Kpn1 +Xho1 digested vector280


5 different clones digest detect:Kpn1 + Xho1

digest :BamH1


24 hours after transfection



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