374-pAd5.CMV-Dest-His
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Cloning of 374-pAd5.CMV-Dest-His
Purpose: to clone pAd5.CMV-Dest-His by gene synthesis
Date: 2011-11-9----
Notebook:Lu yingying
Page:
Clone ID:374
Experimenters: lu yingying
Results
PCR gateway cassette using 446-pVP16 vector as template
ad-GW-F,ad-GW-His-R
cut with Kpn1 + Xho1
ligate to Kpn1 +Xho1 digested vector280
Date: 2011-11-15----
Notebook:Lu yingying
Page:
Clone ID:374
Experimenters: lu yingying
Results
PCR gateway cassette using 446-pVP16 vector as template
ad-GW-F,ad-GW-His-R
cut with Kpn1 + Xho1
ligate to Kpn1 +Xho1 digested vector280
5 different clones digest detect:Kpn1 + Xho1
digest :BamH1
24 hours after transfection
