Transfection reagents comparison 4

Notebook: Zhen Tao #100

Page: 001954

New transfection reagent: 3, 6 and 9 ul/well

PEIMAX: 1, 3 and 5 ul/well

Turbo: 1, 3 and 5 ul/well

Use 50 ul DMEM(-) to prepare each transfection reagent.

Plasmid: 216390 (0.74 ug/ul); 1ug/well; prepared in 50 ul DMEM(-).

Packaging Mix: v:g=1:3 (0.1ug/ul); 3ul/well; add to 50 ul DMEM(-) containing plasmid 216390.

3 day after transfection. --Ztao 11:00, 21 February 2011 (EST)

4 day after transfection. --Ztao 09:19, 22 February 2011 (EST)