1008-pLVX-loxP-sfGFP-loxP

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Cloning of 1008-pLVX-loxP-sfGFP-loxP

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Purpose: Cloning of 1008-pLVX-loxP-sfGFP-loxP by PCR

Date: 2012-2-10--2-15

Notebook:003738

Page:

Clone ID:1008

Experimenters: yingying

Results

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cloning

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Date:2012-2-10


template:254, pAAV.CMV-MCS

primer F:hGHpA-EcoR1-F

primer R:hGHpA-loxP-Xho1-R



 band 1
 band 2

 PCR product
 1007,pLVX-loxP-sfGFP
digest enzymes
 EcoR1 + Xho1


Colony Screening

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Date: 2012- 2-15

clone 2,3,4,5,6 may be right



1008-2 transfection 1-21-2012.JPG1008-3 transfection 1-21-2012.JPG1008-4 transfection 1-21-2012.JPG1008-5 transfection 1-21-2012.JPG1008-6 transfection 1-21-2012.JPG

transfection result: 2-6 positive

Result intepretation/Summary

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Conclusion

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