1008-pLVX-loxP-sfGFP-loxP
Jump to navigation
Jump to search
Cloning of 1008-pLVX-loxP-sfGFP-loxP
[edit]Purpose: Cloning of 1008-pLVX-loxP-sfGFP-loxP by PCR
Date: 2012-2-10--2-15
Notebook:003738
Page:
Clone ID:1008
Experimenters: yingying
Results
[edit]cloning
[edit]Date:2012-2-10
template:254, pAAV.CMV-MCS
primer F:hGHpA-EcoR1-F
primer R:hGHpA-loxP-Xho1-R
| band 1 |
band 2 | |
| PCR product |
1007,pLVX-loxP-sfGFP | |
| digest enzymes |
EcoR1 + Xho1 | |
Colony Screening
[edit]Date: 2012- 2-15
clone 2,3,4,5,6 may be right
transfection result: 2-6 positive
Result intepretation/Summary
[edit]Conclusion
[edit]