1031-pSpeedET-wt-Taq

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Cloning of pSpeedET-wt-Taq (1031)
Purpose: to clone pSpeedET-wt-Taq
Date: 2012-3-21 to 2012-3-23
Notebook: Xiaojing Wang
Page: 004203
Clone ID: 1031
Experimenters: Xiaojing Wang
Results

Digest 462 Wt-Taq-XuYan with Nco1 + Xba1, digest pSpeedET 337 with Nco1 + Xba1.


Ligate, transform top10

Select 4 clones to mini-prep
1031 plasmids are digested by Nco1 + Xba1


Clone 1031-1,-2,-3 may be correct.


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