1031-pSpeedET-wt-Taq

Cloning of pSpeedET-wt-Taq (1031)
Purpose: to clone pSpeedET-wt-Taq
Date: 2012-3-21 to 2012-3-23
Notebook: Xiaojing Wang
Page: 004203
Clone ID: 1031
Experimenters: Xiaojing Wang
Results

Digest 462 Wt-Taq-XuYan with Nco1 + Xba1, digest pSpeedET 337 with Nco1 + Xba1.

Ligate, transform top10

Select 4 clones to mini-prep
1031 plasmids are digested by Nco1 + Xba1

Clone 1031-1,-2,-3 may be correct.