1073-pAd52-CMV5-CuO-DEST-N6His

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Cloning of 1073-pAd52-CMV5-CuO-DEST-N6His

Purpose: Cloning of 1073-pAd52-CMV5-CuO-DEST-N6His

Date: 2012-5-2---2012-5-4

Notebook:0

Page:

Clone ID:1073

Experimenters: yingying

Results

cloning

PCR ccdB cassette

template: 374 pAd5-CMV-Dest-C6XHis

Spe1-6His-GW-F

ad-GW-HA-R2

	band 1	band2
	PCR product	pAd52-CMV5-Cuo-IRES-EGFP, 1070
enzymes	Kpn1 + Nhe1	Spe1 + Nhe1

Colony Screening

Date: 2012-5-4

Make miniprep and digest with Spe1 + Nhe1

no insert

Result intepretation/Summary

Conclusion

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Cloning