1073-pAd52-CMV5-CuO-DEST-N6His
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Cloning of 1073-pAd52-CMV5-CuO-DEST-N6His
[edit]Purpose: Cloning of 1073-pAd52-CMV5-CuO-DEST-N6His
Date: 2012-5-2---2012-5-4
Notebook:0
Page:
Clone ID:1073
Experimenters: yingying
Results
[edit]cloning
[edit]
PCR ccdB cassette
template: 374 pAd5-CMV-Dest-C6XHis
Spe1-6His-GW-F
ad-GW-HA-R2
| band 1 |
band2 | |
| PCR product |
pAd52-CMV5-Cuo-IRES-EGFP, 1070 | |
| enzymes |
Kpn1 + Nhe1 |
Spe1 + Nhe1 |
Colony Screening
[edit]Date: 2012-5-4
Make miniprep and digest with Spe1 + Nhe1
no insert
Result intepretation/Summary
[edit]Conclusion
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