497-pAd52-CMV5-CuO-DEST-C6His
Cloning of 497-pAd52-CMV5-CuO-DEST-C6His
[edit]Purpose: Cloning of 497-pAd52-CMV5-CuO-DEST-C6His
Date: 2012-4-26---2012-4-30
Notebook:0
Page:
Clone ID:497
Experimenters: yingying
Results
[edit]subcloning
[edit]| band 1 |
band 2 | |
| pAd5-CMV-Dest-His 374 |
pAd52-CMV5-Cuo-MCS3, 496 | |
| enzymes |
Kpn1 + Xho1 |
Kpn1 + Sal1 |
Colony Screening
[edit]Date: 2012-4-30
Make miniprep and digest with EcoR1+Mlu1
no insert
Result intepretation/Summary
[edit]
- <br>
Conclusion
[edit]<br>
<br>
<br>
Cloning of 497-pAd52-CMV5-CuO-DEST-C6His
[edit]Purpose: Cloning of 497-pAd52-CMV5-CuO-DEST-C6His
Date: 2012-5-2---2012-5-4
Notebook:0
Page:
Clone ID:497
Experimenters: yingying
Results
[edit]subcloning
[edit]| band 1 |
band 2 | |
| pAd5-CMV-Dest-His 374 |
pAd52-CMV5-Cuo-MCS3, 496 | |
| enzymes |
Kpn1 + Xho1 |
Kpn1 + Sal1 |
Colony Screening
[edit]Date: 2012-5-4
Make miniprep and digest with EcoR1+Mlu1
clone 5,8 may be right
Result intepretation/Summary
[edit]
- &amp;lt;br&amp;gt;
Conclusion
[edit]<br>
<br>
<br>