497-pAd52-CMV5-CuO-DEST-C6His

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Cloning of 497-pAd52-CMV5-CuO-DEST-C6His

[edit]

Purpose: Cloning of 497-pAd52-CMV5-CuO-DEST-C6His

Date: 2012-4-26---2012-4-30

Notebook:0

Page:

Clone ID:497

Experimenters: yingying

Results

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subcloning

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 band 1
 band 2

 pAd5-CMV-Dest-His 374
 pAd52-CMV5-Cuo-MCS3, 496
enzymes
Kpn1 + Xho1
 Kpn1 + Sal1



Colony Screening

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Date: 2012-4-30

Make miniprep and digest with EcoR1+Mlu1

no insert



Result intepretation/Summary

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  • <br>

Conclusion

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<br>


&lt;br&gt;


<br>





Cloning of 497-pAd52-CMV5-CuO-DEST-C6His

[edit]

Purpose: Cloning of 497-pAd52-CMV5-CuO-DEST-C6His

Date: 2012-5-2---2012-5-4

Notebook:0

Page:

Clone ID:497

Experimenters: yingying

Results

[edit]

subcloning

[edit]

File:497


 band 1
 band 2

 pAd5-CMV-Dest-His 374
 pAd52-CMV5-Cuo-MCS3, 496
enzymes
Kpn1 + Xho1
 Kpn1 + Sal1



Colony Screening

[edit]

Date: 2012-5-4

Make miniprep and digest with EcoR1+Mlu1

clone 5,8 may be right



Result intepretation/Summary

[edit]
  • &amp;amp;lt;br&amp;amp;gt;

Conclusion

[edit]

<br>


&lt;br&gt;


<br>




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