6 mRNA Stefania
2012-7-17
1259 and 1260 right size
2012-7-18
1259 and 1260 right
2012-7-20
digest detect : EcoR1+Xho1
| Lane | 1 | 2 | 3 | 4 | 5 | 6 |
| ID | 1256 | 1257 | 1258 | 1259 | 1260 | 1261 |
| insert size should be(bp) | 996 | 1251 | 1926 | 2196 | 360 | 1497 |
2012-7-23
diges again
1----1256
2-----1259
3----1261
2012-7-24
plasmid QC
| lane | 1 | 2 | 3 | 4 | 5 | 6 |
| ID | 1256 | 1257 | 1258 | 1259 | 1260 |
1261 |
2012-7-24
PCR again in 2.5%DMSO: 1256 to 1261
2012-7-27
new primers
1261 no lane
2012-8-8
1327 new clones replace 1261
2012-8-28
1256 ?