1272-pLVX-loxP-TurboRFP-ODC-loxP-TurboGFP

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Cloning of 1272-pLVX-loxP-TurboRFP-ODC-loxP-TurboGFP

[edit]

Purpose: Cloning of 1272-pLVX-loxP-TurboRFP-ODC-loxP-TurboGFP

Date: 2012-8-20---2012-8-22

Notebook:0

Page:

Clone ID:1272

Experimenters: yingying

Results

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cloning

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PCR using 207 pLV-TurboGFP as template, and primers
TurboGFP-Xho1-F cgatcgctcgagGCCACCATGGAGAGCGACGAGAG
TurboGFP-Xba1-R gtctagTCTAGATTATTCTTCACCGGCATCTGCATC


clone into Xho1+ Xba1 digested 1271 pLVX-loxP-TurboRFP-ODC-loxP-TagRFP

1: PCR product

2: vector, 1271 pLVX-loxP-TurboRFP-ODC-loxP-TagRFP




Colony Screening

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Date: 2012-8-22

Make miniprep and digest with Xho1+ Xba1

clone 1,2,3,4,5,6may be right



Result intepretation/Summary

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[edit]
  • <br>

Conclusion

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<br>


<br>




Cloning of 1272-pLVX-loxP-TurboRFP-ODC-loxP-TurboGFP

[edit]

Purpose: Cloning of 1272-pLVX-loxP-TurboRFP-ODC-loxP-TurboGFP

Date: 2012-9-21---2012-9-24

Notebook:0

Page:

Clone ID:1272

Experimenters: yingying

Results

[edit]

cloning

[edit]

PCR using 207 pLV-TurboGFP as template, and primers
TurboGFP-Xho1-F cgatcgctcgagGCCACCATGGAGAGCGACGAGAG
TurboGFP-Xba1-R gtctagTCTAGATTATTCTTCACCGGCATCTGCATC



clone into Xho1+ Xba1 digested 1271 pLVX-loxP-TurboRFP-ODC-loxP-TagRFP

1: PCR product

2: vector, 1271 pLVX-loxP-TurboRFP-ODC-loxP-TagRFP




Colony Screening

[edit]

Date: 2012-9-23

Make miniprep and digest with Xho1+ Xba1

no insert




Result intepretation/Summary

[edit]

[edit]
  • &amp;amp;lt;br&amp;amp;gt;

Conclusion

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<br>


<br>





= Cloning of 1272-pLVX-loxP-TurboRFP-ODC-loxP-TurboGFP

[edit]

Purpose: Cloning of 1272-pLVX-loxP-TurboRFP-ODC-loxP-TurboGFP

Date: 2012-9-27---

Notebook:0

Page:

Clone ID:1272

Experimenters: yingying

Results

[edit]

cloning

[edit]




 band 1
 band 2

 1402,pLVX-TurboGFP
 1271,pLVX-loxP-TurboRFP-ODC-loxP-TagRFP
enzymes
             Xho1+ Xba1



Colony Screening

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Date: 2012/9/29


digest plasmid with Xho1+ Xba1





10/7/2012

Digest with Xba1+Xho1

Transfection 10/9/2012, 10 ul miniprep DNA

photo on 10/10/2012, 24 hrs after transfection

grow clone # 2 for midiprep-->stable cell line

Result intepretation/Summary

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  • &lt;br&gt;

Conclusion

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&lt;br&gt;


&lt;br&gt;


<br>




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