1272-pLVX-loxP-TurboRFP-ODC-loxP-TurboGFP
Cloning of 1272-pLVX-loxP-TurboRFP-ODC-loxP-TurboGFP
[edit]Purpose: Cloning of 1272-pLVX-loxP-TurboRFP-ODC-loxP-TurboGFP
Date: 2012-8-20---2012-8-22
Notebook:0
Page:
Clone ID:1272
Experimenters: yingying
Results
[edit]cloning
[edit]PCR using 207 pLV-TurboGFP as template, and primers
TurboGFP-Xho1-F cgatcgctcgagGCCACCATGGAGAGCGACGAGAG
TurboGFP-Xba1-R gtctagTCTAGATTATTCTTCACCGGCATCTGCATC
clone into Xho1+ Xba1 digested 1271 pLVX-loxP-TurboRFP-ODC-loxP-TagRFP
1: PCR product
2: vector, 1271 pLVX-loxP-TurboRFP-ODC-loxP-TagRFP
Colony Screening
[edit]Date: 2012-8-22
Make miniprep and digest with Xho1+ Xba1
clone 1,2,3,4,5,6may be right
Result intepretation/Summary
[edit]- <br>
Conclusion
[edit]<br>
<br>
Cloning of 1272-pLVX-loxP-TurboRFP-ODC-loxP-TurboGFP
[edit]Purpose: Cloning of 1272-pLVX-loxP-TurboRFP-ODC-loxP-TurboGFP
Date: 2012-9-21---2012-9-24
Notebook:0
Page:
Clone ID:1272
Experimenters: yingying
Results
[edit]cloning
[edit]PCR using 207 pLV-TurboGFP as template, and primers
TurboGFP-Xho1-F cgatcgctcgagGCCACCATGGAGAGCGACGAGAG
TurboGFP-Xba1-R gtctagTCTAGATTATTCTTCACCGGCATCTGCATC
clone into Xho1+ Xba1 digested 1271 pLVX-loxP-TurboRFP-ODC-loxP-TagRFP
1: PCR product
2: vector, 1271 pLVX-loxP-TurboRFP-ODC-loxP-TagRFP
Colony Screening
[edit]Date: 2012-9-23
Make miniprep and digest with Xho1+ Xba1
no insert
Result intepretation/Summary
[edit]- &amp;lt;br&amp;gt;
Conclusion
[edit]<br>
<br>
|
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= Cloning of 1272-pLVX-loxP-TurboRFP-ODC-loxP-TurboGFP
[edit]Purpose: Cloning of 1272-pLVX-loxP-TurboRFP-ODC-loxP-TurboGFP
Date: 2012-9-27---
Notebook:0
Page:
Clone ID:1272
Experimenters: yingying
Results
[edit]cloning
[edit]
| band 1 |
band 2 | |
| 1402,pLVX-TurboGFP |
1271,pLVX-loxP-TurboRFP-ODC-loxP-TagRFP | |
| enzymes |
Xho1+ Xba1 | |
Colony Screening
[edit]Date: 2012/9/29
digest plasmid with Xho1+ Xba1
10/7/2012
Digest with Xba1+Xho1
Transfection 10/9/2012, 10 ul miniprep DNA
photo on 10/10/2012, 24 hrs after transfection
grow clone # 2 for midiprep-->stable cell line
Result intepretation/Summary
[edit]- <br>
Conclusion
[edit]<br>
<br>
<br>