1428-pT7-MBP

Cloning of 1428-pT7-MBP

Purpose: Cloning of 1428-pT7-MBP

Date: 2012-10-18---2012-10-20

Notebook:0

Page:

Clone ID:1428

Experimenters: yingying

Results

subcloning

digest 1280 pT7 with Xba1 + EcoR1---> vector
digest pDB.His.MBP or pDB.His.MBP.amp(lane 3,4,5) with Xba1 + EcoR1--->insert
ligate and transform

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Colony Screening

Date: 2012-10-20

Make miniprep and digest with Xba1 + EcoR1

clone1 2 3 5right

Result intepretation/Summary

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Conclusion

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