1428-pT7-MBP
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Cloning of 1428-pT7-MBP
[edit]Purpose: Cloning of 1428-pT7-MBP
Date: 2012-10-18---2012-10-20
Notebook:0
Page:
Clone ID:1428
Experimenters: yingying
Results
[edit]subcloning
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digest 1280 pT7 with Xba1 + EcoR1---> vector
digest pDB.His.MBP or pDB.His.MBP.amp(lane 3,4,5) with Xba1 + EcoR1--->insert
ligate and transform
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Colony Screening
[edit]Date: 2012-10-20
Make miniprep and digest with Xba1 + EcoR1
clone1 2 3 5right
Result intepretation/Summary
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Conclusion
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