1429-pT7A3-MBP

From ELN
Revision as of 08:59, 22 October 2012 by Paul.xu (talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to navigation Jump to search

Cloning of 1429-pT7A3-MBP

[edit]

Purpose: Cloning of 1429-pT7A3-MBP

Date: 2012-10-18---2012-10-20

Notebook:0

Page:

Clone ID:1429

Experimenters: yingying

Results

[edit]

subcloning

[edit]


digest 1279 pT7A3 (v)with Xba1 + EcoR1---> vector
digest pDB.His.MBP or pDB.His.MBP.amp(lane3,4,5) with Xba1 + EcoR1--->insert
ligate and transform


Colony Screening

[edit]

Date: 2012-10-20

Make miniprep and digest with Xba1 + EcoR1

clone1,2,3,4,5 right

Result intepretation/Summary

[edit]
  • <br>

Conclusion

[edit]

<br>


<br>


<br>


<br>




Retrieved from "https://eln.excellgen.com/index.php?title=1429-pT7A3-MBP&oldid=6652"