1325-pDBHisMBP-gpI3L

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Cloning of 1325-pDBHisMBP-gpI3L

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Purpose: Cloning of 1325-pDBHisMBP-gpI3L

Date: 2012-10-25---

Notebook:0

Page:

Clone ID:1325

Experimenters: yingying

Results

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cloning

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PCR genomic DNA (Vaccinia virus strain WR, ATCC)

VVgpI3L-Nde1-F
tagcaccatatgagtaaggtaatcaagaag
VVgpI3L-Xho1-R




   lane 1
   lane 2

 PCR product
 360,pDB.His.MBP
enzymes
            Nde1 + Xho1



Colony Screening

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Date: 2012-

Make miniprep and digest with



Result intepretation/Summary

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  • <br>

Conclusion

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