1325-pDBHisMBP-gpI3L
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Cloning of 1325-pDBHisMBP-gpI3L
[edit]Purpose: Cloning of 1325-pDBHisMBP-gpI3L
Date: 2012-10-25---
Notebook:0
Page:
Clone ID:1325
Experimenters: yingying
Results
[edit]cloning
[edit]
PCR genomic DNA (Vaccinia virus strain WR, ATCC)
VVgpI3L-Nde1-F
tagcaccatatgagtaaggtaatcaagaag
VVgpI3L-Xho1-R
| lane 1 |
lane 2 | |
| PCR product |
360,pDB.His.MBP | |
| enzymes |
Nde1 + Xho1 | |
Colony Screening
[edit]Date: 2012-
Make miniprep and digest with
Result intepretation/Summary
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Conclusion
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