PDB-His-NusA-StrepII

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Primer-F

StrepII-T: agcttCTGCAGctcgagTGGAGCCACCCGCAGTTCGAAAAGTgAa

Primer-R

StrepII-B: tcgatTcACTTTTCGAACTGCGGGTGGCTCCActcgagCTGCAGa

anneal these two oligos and ligate to HindIII and Xho1 digested pDB.His.NusA. Transform Top 10 competent cells



mini-prep digest with Xba1+Pst1


--Paul.xu 17:24, 20 April 2011 (EST)

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