1427-pLVX-Kozak-loxP-TurboRFP-ODC-loxP-TurboGFP

From ELN
Revision as of 11:48, 20 November 2012 by Paul.xu (talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to navigation Jump to search

Cloning of 1427-pLVX-Kozak-loxP-TurboRFP-ODC-loxP-TurboGFP

[edit]

Purpose: Cloning of 1427-pLVX-Kozak-loxP-TurboRFP-ODC-loxP-TurboGFP

Date: 2012-11-8---2012-11-14

Notebook:0

Page:

Clone ID:1427

Experimenters: yingying

Results

[edit]

cloning

[edit]


PCR using 81 TurboGFP as template, and
the new TurboGFP-Xho1-F (without Kozak seqeunce and start codon)
and
TurboGFP-Xba1-R


lane
 1
 2

 PCR product
 1425-pLVX-Kozak-loxP-TurboRFP-ODC-loxP-Gluc
enzymes
           Xho1+ Xba1


Colony Screening

[edit]

Date: 2012-11-13

Make miniprep and digest with  Xho1+ Xba1



2012-11-14

6 clonies PCR screening:new TurboGFP-Xho1-F+PGK-P-R-Seq

clone 3,4,5,6 may be right ,need to sequence



Result intepretation/Summary

[edit]
  • <br>

Conclusion

[edit]

<br>






Cloning of 1427-pLVX-Kozak-loxP-TurboRFP-ODC-loxP-TurboGFP

[edit]

Purpose: Cloning of 1427-pLVX-Kozak-loxP-TurboRFP-ODC-loxP-TurboGFP

Date: 2012-11-15---2012-11-18

Notebook:0

Page:

Clone ID:1427

Experimenters: yingying

Results

[edit]

cloning

[edit]


PCR loxP-TurboRFP-ODC-loxP from 1271 pLVX-loxP-TurboRFP-ODC-loxP-Gluc

LoxP-TurboRFP-BamH1-F1+LoxP-TurboRFP-BamH1-F2+LoxP-hGHpA-Xho1-R2





lane
 1
 2

 PCR product
 1427-pLVX-Kozak-loxP-TurboRFP-ODC-loxP-TurboGFP
enzymes
           BamH1 + Xho1


Colony Screening

[edit]

Date: 2012-11-19

Make miniprep and digest with  BamH1 + Xho1


Clone 1,2,3,4,5,6,7 need to sequencing


Result intepretation/Summary

[edit]
  • <br>

Conclusion

[edit]

<br>






Retrieved from "https://eln.excellgen.com/index.php?title=1427-pLVX-Kozak-loxP-TurboRFP-ODC-loxP-TurboGFP&oldid=7127"