1427-pLVX-Kozak-loxP-TurboRFP-ODC-loxP-TurboGFP
Cloning of 1427-pLVX-Kozak-loxP-TurboRFP-ODC-loxP-TurboGFP
[edit]Purpose: Cloning of 1427-pLVX-Kozak-loxP-TurboRFP-ODC-loxP-TurboGFP
Date: 2012-11-8---2012-11-14
Notebook:0
Page:
Clone ID:1427
Experimenters: yingying
Results
[edit]cloning
[edit]
PCR using 81 TurboGFP as template, and
the new TurboGFP-Xho1-F (without Kozak seqeunce and start codon)
and
TurboGFP-Xba1-R
| lane |
1 |
2 |
| PCR product |
1425-pLVX-Kozak-loxP-TurboRFP-ODC-loxP-Gluc | |
| enzymes |
Xho1+ Xba1 | |
Colony Screening
[edit]Date: 2012-11-13
Make miniprep and digest with Xho1+ Xba1
2012-11-14
6 clonies PCR screening:new TurboGFP-Xho1-F+PGK-P-R-Seq
clone 3,4,5,6 may be right ,need to sequence
Result intepretation/Summary
[edit]- <br>
Conclusion
[edit]<br>
Cloning of 1427-pLVX-Kozak-loxP-TurboRFP-ODC-loxP-TurboGFP
[edit]Purpose: Cloning of 1427-pLVX-Kozak-loxP-TurboRFP-ODC-loxP-TurboGFP
Date: 2012-11-15---2012-11-18
Notebook:0
Page:
Clone ID:1427
Experimenters: yingying
Results
[edit]cloning
[edit]
PCR loxP-TurboRFP-ODC-loxP from 1271 pLVX-loxP-TurboRFP-ODC-loxP-Gluc
LoxP-TurboRFP-BamH1-F1+LoxP-TurboRFP-BamH1-F2+LoxP-hGHpA-Xho1-R2
| lane |
1 |
2 |
| PCR product |
1427-pLVX-Kozak-loxP-TurboRFP-ODC-loxP-TurboGFP | |
| enzymes |
BamH1 + Xho1 | |
Colony Screening
[edit]Date: 2012-11-19
Make miniprep and digest with BamH1 + Xho1
Clone 1,2,3,4,5,6,7 need to sequencing
Result intepretation/Summary
[edit]- <br>
Conclusion
[edit]<br>