1026-1028-pET28b lambda exo beta gam

Cloning of 1026~1028, pET28b lambda alpha (exo), beta, gama

Purpose: Cloning of lambda RED (exo and related proteins)

Date: 2012-3-25-

Notebook:

Page:

Clone ID:1026

Experimenters: yingying

Results

cloning

date: 3/25/2012

PCR using phage DNA

3/28/2012

Miniprep DigestionNde1+ Xho1

sequencing: L1-3 (no priming), L2-1 (bad quality), L3-5 (too many mutations)

L1 is lambda alpha, 650bp

L2 is lambda gama, 400 bp

L3 is lamda beta, 700bp

3/29/2012, colony PCR screening

1026_1530_1533_1565pcr.JPG‎

3/30/2012Make new miniprep, digestion with Nde1+ Xho1

1026_1530_1533_1565pcr.JPG‎ 1026_1530_1533_1565pcr.JPG‎

exo:  left 4

bet: middle 4

gam: right 4

send sequencing, left 1, middle 2, right 2

4/10/2012 select 4 clones to mini-prep, digest

Cloning of 1026 pET28b-LambdaExo

cloning

Purpose: Cloning of 1026 pET28b-LambdaExo

Date: 2013-1-3-

Notebook:

Page:

Clone ID:1026

Experimenters: Shanyi

Results

cloning

PCR using phage DNA (25cycles)

colony screening

Miniprep Digestion Nde1+ Xho1