1026-1028-pET28b lambda exo beta gam
Cloning of 1026~1028, pET28b lambda alpha (exo), beta, gama
[edit]Purpose: Cloning of lambda RED (exo and related proteins)
Date: 2012-3-25-
Notebook:
Page:
Clone ID:1026
Experimenters: yingying
Results
[edit]cloning
[edit]date: 3/25/2012
PCR using phage DNA
3/28/2012
Miniprep DigestionNde1+ Xho1
sequencing: L1-3 (no priming), L2-1 (bad quality), L3-5 (too many mutations)
L1 is lambda alpha, 650bp
L2 is lambda gama, 400 bp
L3 is lamda beta, 700bp
3/29/2012, colony PCR screening
3/30/2012Make new miniprep, digestion with Nde1+ Xho1
1026_1530_1533_1565pcr.JPG 1026_1530_1533_1565pcr.JPG 
exo: left 4
bet: middle 4
gam: right 4
send sequencing, left 1, middle 2, right 2
4/10/2012 select 4 clones to mini-prep, digest
Cloning of 1026 pET28b-LambdaExo
[edit]cloning
[edit]Purpose: Cloning of 1026 pET28b-LambdaExo
Date: 2013-1-3-
Notebook:
Page:
Clone ID:1026
Experimenters: Shanyi
Results
[edit]cloning
[edit]PCR using phage DNA (25cycles)
colony screening
[edit]
Miniprep Digestion Nde1+ Xho1
