1044 pET28b-T4-DNA Pol

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Cloning of 1044 pET28b-T4-DNA Pol

[edit]

Purpose: Cloning of 1044 pET28b-T4-DNA Pol

Date: Jan , 2013

Notebook: 

Page:

Clone ID: 1044

Experimenters: Shanyi

Results

[edit]

Cloning

[edit]

PCR

50oc 30s, 65oc 5min, 30cycle


Colony screen

[edit]


Cloning of 1044 pET28b-T4-DNA Pol

[edit]

Purpose: Cloning of 1044 pET28b-T4-DNA Pol

Date: Jan , 2013

Notebook: 

Page:

Clone ID: 1044

Experimenters: Shanyi

Results

[edit]

Cloning

[edit]

PCR


use first round pcr as template, 52oc 30s,72oc 3min,29cycle.


digest with NheI and Sal1

Colony screen

[edit]

digest with NheI and Sal1

 



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