1044 pET28b-T4-DNA Pol
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Cloning of 1044 pET28b-T4-DNA Pol
[edit]Purpose: Cloning of 1044 pET28b-T4-DNA Pol
Date: Jan , 2013
Notebook:
Page:
Clone ID: 1044
Experimenters: Shanyi
Results
[edit]Cloning
[edit]PCR
50oc 30s, 65oc 5min, 30cycle
Colony screen
[edit]
Cloning of 1044 pET28b-T4-DNA Pol
[edit]Purpose: Cloning of 1044 pET28b-T4-DNA Pol
Date: Jan , 2013
Notebook:
Page:
Clone ID: 1044
Experimenters: Shanyi
Results
[edit]Cloning
[edit]PCR
use first round pcr as template, 52oc 30s,72oc 3min,29cycle.
digest with NheI and Sal1
Colony screen
[edit]digest with NheI and Sal1
