1601 pT7A3-6His-GST-i3C

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Cloning of 1601 pT7A3-6His-GST-i3C

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Purpose: Cloning of 1601 pT7A3-6His-GST-i3C

Date: 2013-1-28---2013-1-30

Notebook:0

Page:

Clone ID:1601

Experimenters: Shanyi

Results

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subcloning

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subclone i3C from 1554 pT7A3-MBP-i3C to 1597 pT7A3-6XHis.GST using Nde1 + Xho1


 band 1
 band 2

 1597 pT7A3-6XHis.GST
 1554 pT7A3-MBP-i3C
enzymes
             Nde1 + Xho1

Colony Screening

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Date: 2013-1-30

Make miniprep and digest with Nde1 + Xho1.

digest positive clone with Nco1 alone to confirm GST not MBP is in the construct. Nco1 will cut out one band from MBP




Conclusion

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Protein Expression

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1625_pCold-GST-(3c)-KEAP1#Protein_Expression

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