1601 pT7A3-6His-GST-i3C
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Cloning of 1601 pT7A3-6His-GST-i3C
[edit]Purpose: Cloning of 1601 pT7A3-6His-GST-i3C
Date: 2013-1-28---2013-1-30
Notebook:0
Page:
Clone ID:1601
Experimenters: Shanyi
Results
[edit]subcloning
[edit]subclone i3C from 1554 pT7A3-MBP-i3C to 1597 pT7A3-6XHis.GST using Nde1 + Xho1
| band 1 |
band 2 | |
| 1597 pT7A3-6XHis.GST |
1554 pT7A3-MBP-i3C | |
| enzymes |
Nde1 + Xho1 | |
Colony Screening
[edit]Date: 2013-1-30
Make miniprep and digest with Nde1 + Xho1.
digest positive clone with Nco1 alone to confirm GST not MBP is in the construct. Nco1 will cut out one band from MBP
