1740 pT7A3-8HZB2

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Cloning of 1740 pT7A3-8HZB2

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Purpose: Cloning of1740 pT7A3-8HZB2

Date: 2013-5---

Notebook:0

Page:

Clone ID:1740

Experimenters: Shanyi

Results

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cloning

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PCR Zbasic2 from 1705 pT7A3-ZB2-T4Lysozyme using

6H-ZB2-Nco1-F atcgac CCATGGGCAGCAGCCATCATCATCATCATCAC cat cac gtcgacaacaagttcaacaag

ZB2-E3C-Nde1-R (ctgaacgacgcgcagccgaag ctggaagttctgttccaggggccg catatg ctagca) tgctagcatatgcggcccctggaacagaacttccagcttcggctgcgcgtcgttcag


digest with Nco1 + Nde1



Colony Screening

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Date: 2013-5-20

Make miniprep and digest with Nco1 + Nde1



Result intepretation/Summary

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Conclusion

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