1740 pT7A3-8HZB2
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Cloning of 1740 pT7A3-8HZB2
[edit]Purpose: Cloning of1740 pT7A3-8HZB2
Date: 2013-5---
Notebook:0
Page:
Clone ID:1740
Experimenters: Shanyi
Results
[edit]cloning
[edit]PCR Zbasic2 from 1705 pT7A3-ZB2-T4Lysozyme using
6H-ZB2-Nco1-F atcgac CCATGGGCAGCAGCCATCATCATCATCATCAC cat cac gtcgacaacaagttcaacaag
ZB2-E3C-Nde1-R (ctgaacgacgcgcagccgaag ctggaagttctgttccaggggccg catatg ctagca) tgctagcatatgcggcccctggaacagaacttccagcttcggctgcgcgtcgttcag
digest with Nco1 + Nde1
Colony Screening
[edit]Date: 2013-5-20
Make miniprep and digest with Nco1 + Nde1
