1750 pT7A3-Tks

From ELN
Revision as of 17:33, 3 June 2013 by Paul.xu (talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to navigation Jump to search

Cloning of 1750 pT7A3-Tks

[edit]

Purpose: Cloning of 1750 pT7A3-Tks

Date: May , 2013

Notebook: 

Page:

Clone ID: 1750

Experimenters: Shanyi

Results

[edit]

Cloning

[edit]

subclone from 1265 to 1279

1279 digest with NdeI +XhoI

1265 digest with Nde1 + Xho1(should get three bands, because there is one XhoI site in TKS, cut out the lower two bands)


 Colony screen

[edit]

digest with Nde1 + Xho1 

DIGEST_WITH_HindIII ,SHOULD GET ONE BAND

DIGEST WITH NdeI+KpnI

Result intepretation/Summary

[edit]

Conclusion

[edit]
Retrieved from "https://eln.excellgen.com/index.php?title=1750_pT7A3-Tks&oldid=9348"