1750 pT7A3-Tks
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Cloning of 1750 pT7A3-Tks
[edit]Purpose: Cloning of 1750 pT7A3-Tks
Date: May , 2013
Notebook:
Page:
Clone ID: 1750
Experimenters: Shanyi
Results
[edit]Cloning
[edit]subclone from 1265 to 1279
1279 digest with NdeI +XhoI
1265 digest with Nde1 + Xho1(should get three bands, because there is one XhoI site in TKS, cut out the lower two bands)
Colony screen
[edit]digest with Nde1 + Xho1
DIGEST_WITH_HindIII ,SHOULD GET ONE BAND
DIGEST WITH NdeI+KpnI
