Mammalian cell lysis buffer

50 mM Tris/HCl, pH7.5

150 mM NaCl

1% Nonidet P-40 (IGEPAL CA-630; Sigma)

0.1% sodium azide

1 mM NaF

2 mM Na3VO4 1 tablet/10 mL of protease inhibitors mixture tablets (Roche)