Mammalian cell lysis buffer
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50 mM Tris/HCl, pH7.5
150 mM NaCl
1% Nonidet P-40 (IGEPAL CA-630; Sigma)
0.1% sodium azide
1 mM NaF
2 mM Na3VO4 1 tablet/10 mL of protease inhibitors mixture tablets (Roche)