1772 pCMV5-hIgG-Lc-NRF2

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Cloning of1772 pCMV5-hIgG-Lc-NRF2

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Purpose: Cloning of 1772 pCMV5-hIgG-Lc-NRF2

Date: 2013-7--9-

Notebook:05846

Page:

Clone ID:1772

Experimenters: Shanyi

Results

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cloning

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PCR using 1622 pLX304-NRF2 as template


digest PCR products with Nhe1 + Kpn1,

then ligate to Nhe1 + Kpn1 digested 1590 pCMV5-hIgG-Lc-TagGFP-Fc-no-intron-(3c)-22nt-IRES-TagRFP-WPRE-OriP



Colony Screening

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Date: 2013-7-

Make miniprep and digest Nhe1 + Kpn1


Cloning of1772 pCMV5-hIgG-Lc-NRF2

[edit]

Purpose: Cloning of 1772 pCMV5-hIgG-Lc-NRF2

Date: 2013-7--9-

Notebook:05846

Page:

Clone ID:1772

Experimenters: Shanyi

Results

[edit]

cloning

[edit]

PCR using 1622 pLX304-NRF2 as template(USE NRF2-Nde1-Nhe1-F2)


digest PCR products with Nhe1 + Kpn1,

then ligate to Nhe1 + Kpn1 digested 1590 pCMV5-hIgG-Lc-TagGFP-Fc-no-intron-(3c)-22nt-IRES-TagRFP-WPRE-OriP



Colony Screening

[edit]

Date: 2013-7-

Make miniprep and digest EcoRI + Kpn1





Result intepretation/Summary

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Conclusion

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