1772 pCMV5-hIgG-Lc-NRF2
Cloning of1772 pCMV5-hIgG-Lc-NRF2
[edit]Purpose: Cloning of 1772 pCMV5-hIgG-Lc-NRF2
Date: 2013-7--9-
Notebook:05846
Page:
Clone ID:1772
Experimenters: Shanyi
Results
[edit]cloning
[edit]PCR using 1622 pLX304-NRF2 as template
digest PCR products with Nhe1 + Kpn1,
then ligate to Nhe1 + Kpn1 digested 1590 pCMV5-hIgG-Lc-TagGFP-Fc-no-intron-(3c)-22nt-IRES-TagRFP-WPRE-OriP
Colony Screening
[edit]Date: 2013-7-
Make miniprep and digest Nhe1 + Kpn1
Cloning of1772 pCMV5-hIgG-Lc-NRF2
[edit]Purpose: Cloning of 1772 pCMV5-hIgG-Lc-NRF2
Date: 2013-7--9-
Notebook:05846
Page:
Clone ID:1772
Experimenters: Shanyi
Results
[edit]cloning
[edit]PCR using 1622 pLX304-NRF2 as template(USE NRF2-Nde1-Nhe1-F2)
digest PCR products with Nhe1 + Kpn1,
then ligate to Nhe1 + Kpn1 digested 1590 pCMV5-hIgG-Lc-TagGFP-Fc-no-intron-(3c)-22nt-IRES-TagRFP-WPRE-OriP
Colony Screening
[edit]Date: 2013-7-
Make miniprep and digest EcoRI + Kpn1
