1783-pT7A31

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Cloning of 1783-pT7A31

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Purpose: Cloning of 1783-pT7A31

Date: 2013-7-17---2013-7-19

Notebook:006026

Page:

Clone ID:1783

Experimenters: yingying

Results

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cloning

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lane
 5

 651,pT7A30-IFN-gamma
enzymes
 Nde1+Xho1

digest vector with Nde1 + Xho1,
Ligate to annealed oligos
Nde1-Sal1-T+Nde1-Sal1-B


Colony Screening

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Date: 2013-7-19

Make miniprep and digest with HindIII + Sma1, should get one band (lineared, HindIII is gone)




Result intepretation/Summary

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Conclusion

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