1783-pT7A31

Cloning of 1783-pT7A31

Purpose: Cloning of 1783-pT7A31

Date: 2013-7-17---2013-7-19

Notebook:006026

Page:

Clone ID:1783

Experimenters: yingying

Results

cloning

lane	5
	651,pT7A30-IFN-gamma
enzymes	Nde1+Xho1

digest vector with Nde1 + Xho1,
Ligate to annealed oligos
Nde1-Sal1-T+Nde1-Sal1-B

Colony Screening

Date: 2013-7-19

Make miniprep and digest with HindIII + Sma1, should get one band (lineared, HindIII is gone)