1498 1797

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Protein Purification

[edit]

2013/8/2
SHANYI


20ul sample from 1498 fraction16,19,24,33,37 and 1797fraction16,18,22,31,41.

Lane
 Name
 Protein Name
 Clone ID
 Host
 Purification
 pI/Mw
 Expression
1
 pT7A3-TaqDNALigase
 TaqDNALigase
   1498
 Nico21DE3plysN

10 ml Ni column

Bio-Sil TSK 250

76KD
  yes



2
3
4
5
6








8


Cold-6His-3C-MBP

 3C-MBP


1797

 

BL21(DE3)Star, pRare2-pLysS
 

10 ml Ni column

Bio-Sil TSK 250

   6.01 / 62696.31
 



 

9
10
11
12
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