1498 1797
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Protein Purification
[edit]2013/8/2
SHANYI
20ul sample from 1498 fraction16,19,24,33,37 and 1797fraction16,18,22,31,41.
| Lane |
Name |
Protein Name |
Clone ID |
Host |
Purification |
pI/Mw |
Expression |
| 1 |
pT7A3-TaqDNALigase |
TaqDNALigase |
1498 |
Nico21DE3plysN |
10 ml Ni column Bio-Sil TSK 250 |
76KD |
yes |
| 2 | |||||||
| 3 | |||||||
| 4 | |||||||
| 5 | |||||||
| 6 | |||||||
| 8 |
Cold-6His-3C-MBP |
3C-MBP |
1797 |
BL21(DE3)Star, pRare2-pLysS |
10 ml Ni column
Bio-Sil TSK 250 |
6.01 / 62696.31 |
|
| 9 | |||||||
| 10 | |||||||
| 11 | |||||||
| 12 |