1007-pLVX-loxP-sfGFP
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Cloning of 1007(548)-pLVX-loxP-sfGFP
[edit]Purpose: Cloning of 1007-pLVX-loxP-sfGFP from 535-1 pAAV-CMV-DIO-sfGFP to PLVX-plus2,164
Date: 2012-2-7--
Notebook:003732
Page:
Clone ID:1007
Experimenters: yingying
NOTE: change 548 into 1007
Results
[edit]Subcloning
[edit]Date:2012-2-7
| band 1 |
band 2 | |
| 535-1 pAAV-CMV-DIO-sfGFP |
PLVX-plus2,164 | |
| digest enzymes |
EcoR1 + BamH1 | |
Colony Screening
[edit]Date: 2012-2-9
Make miniprep and digest with EcoR1 + BamH1
clone 1 to 8 may be right, need to transfect into cells
transfection result: all positive
Result intepretation/Summary
[edit]Conclusion
[edit]