1009-pLVX-TurboRFP
Cloning of 1009-pLVX-TurboRFP
[edit]Purpose: Cloning of 1009-pLVX-TurboRFP by genesynthesis
Date: 2012-2-10--2-15
Notebook:003738
Page:
Clone ID:1009
Experimenters: yingying
Results
[edit]cloning
[edit]Date:2012-2-10
template:TurboRFP-1 to TurboRFP-20 mixture
primer F:TurboRFP-1
primer R:TurboRFP-20
| band 3 |
band 4 | |
| PCR product |
pLVX-Plus2 164 | |
| digest enzymes |
Xho1 + Xba1 | |
Colony Screening
[edit]Date: 2012-2-15
Make miniprep and digest with Xho1 + Xba1
Result intepretation/Summary
[edit]Conclusion
[edit]
Cloning of 1009-pLVX-TurboRFP
[edit]Purpose: Cloning of 1009-pLVX-TurboRFP by genesynthesis
Date: 2012-2-16--
Notebook:003745--
Page:
Clone ID:1009
Experimenters: yingying
Results
[edit]cloning
[edit]Date:2012-2-10
template:TurboRFP-1 to TurboRFP-20 mixture
primer F:TurboRFP-1
primer R:TurboRFP-20
| band 1 |
band 2 | |
| PCR product |
pLVX-Plus2 164 | |
| digest enzymes |
Xho1 + Xba1 | |
Colony Screening
[edit]Date: 2012
Make miniprep and digest with Xho1 + Xba1
no insert
Result intepretation/Summary
[edit]Conclusion
[edit]
Cloning of 1009-pLVX-TurboRFP
[edit]Purpose: Cloning of 1009-pLVX-TurboRFP by genesynthesis
Date: 2012-3-13--2012-3-15
Notebook:
Page:
Clone ID:1009
Experimenters: yingying
Results
[edit]cloning
[edit]Date:2012-3-15
mix 1020 TurboRFP-TA vector 8clonies insert together,and ligate to digested pLVX-Plus2 164
Colony Screening
Date: 2012-3-15
Make miniprep and digest with Xho1 + Xba1
no insert
Result intepretation/Summary
[edit]Conclusion
[edit]