1011-pET30b-FluASeg-7
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Cloning of 1011-pET30b-FluASeg-7
[edit]Purpose: Cloning of 1005-pBad24-Tat-sfGFP by PCR
Date: 2012-3-21--2012-
Notebook:
Page:
Clone ID:1010
Experimenters: yingying
Results
[edit]cloning
[edit]Date:2012-3-22
template:Influenza A virus (A/WSN/1933(H1N1)) segment 7
FluASeg-7-NdeI-F
actagc CAT ATG CAC CAT CAT CAT CAT CAT agtcttctaaccgaggtc
FluASeg-7-Xho1-R
(catcggtctcataggcaaatgCTCGAG atcaga)
tctgatCTCGAGcatttgcctatgagaccgatg
| band 1 |
band 2 | |
| PCR product |
pET30b | |
| digest enzymes |
Nde1 + Xho1 | |
Colony Screening
[edit]Date: 2012-3-26
Make miniprep and digest with Nde1 + Xho1
clone 1,2,3,4 may be right
Protein expression in Nico21(DE3)
Result intepretation/Summary
[edit]- <br>
Conclusion
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