1017-pDNR-Dual-ATR-C-His
Cloning of 1017-pDNR-Dual-ATR-C-His
[edit]Purpose: Cloning of 1005-pBad24-Tat-sfGFP by PCR
Date: 2012-3-21--2012-3-26
Notebook:0
Page:
Clone ID:1017
Experimenters: yingying
Results
[edit]cloning
[edit]Date:2012-3-21
Template:1016 pDNR-Dual-ATR
ATR-AgeI-F
GCAATAAACCGGTTGATGGAAG
ATR-BssHII-R BamH1 BssHII
(GGTTGGACTCCATATATGcatcatcaccatcaccatTAGGGATCCGCGCGCagctaca)
tgtagctGCGCGCGGATCCCTAatggtgatggtgatgatgCATATATGGAGTCCAACC
| band 3 |
band 4 | |
| PCR prodduct |
pDNR-Dual-ATR | |
| enzymes |
AgeI+BssHII | |
Colony Screening
[edit]Date: 2012-3-26
Make miniprep and digest with AgeI+BssHII
6,7,8,9,10 no insert ,need to clone again
Result intepretation/Summary
- <br>
Conclusion
[edit]<br>
<br>
<br>
Cloning of 1017-pDNR-Dual-ATR-C-His
[edit]Purpose: Cloning of 1005-pBad24-Tat-sfGFP by PCR
Date: 2012-3-27--2012-3-30
Notebook:
Page:003783
Clone ID:1017
Experimenters: yingying
Results
[edit]cloning
[edit]Date:2012-3-21
Template:1016 pDNR-Dual-ATR
ATR-AgeI-F
GCAATAAACCGGTTGATGGAAG
ATR-BssHII-R BamH1 BssHII
(GGTTGGACTCCATATATGcatcatcaccatcaccatTAGGGATCCGCGCGCagctaca)
tgtagctGCGCGCGGATCCCTAatggtgatggtgatgatgCATATATGGAGTCCAACC
digest:
AgeI at 37c for 1 hour,then BssHII at 50c for 1 hour
| band 3 |
band 4 | |
| PCR prodduct |
pDNR-Dual-ATR | |
| enzymes |
AgeI+BssHII | |
Colony Screening
[edit]Date: 2012-3-29
Make miniprep and digest with Xba1
no band
Result intepretation/Summary
- <br>
Conclusion
[edit]<br>
<br>
<br>
Cloning of 1017-pDNR-Dual-ATR-C-His
[edit]Purpose: Cloning of 1017-pDNR-Dual-ATR-C-His
Date: 2012-4-13---2012-4-16
Notebook:0
Page:
Clone ID:1017
Experimenters: yingying
Results
[edit]cloning
[edit]
template:1016 pDNR-Dual-ATR
PCR using these primers
ATR-AgeI-F
GCAATAAACCGGTTGATGGAAG
ATR-AvrII-R BamH1 AvrII
(GGTTGGACTCCATATATGcatcatcaccatcaccatTAGGGATCCTAGGagctacaa)
ttgtagctCCTAGGATCCCTAatggtgatggtgatgatgCATATATGGAGTCCAACC
vector can not de digested
| band 1 |
band 2 | |
| PCR product |
1016,pDNR-Dual-ATR | |
| enzymes |
AgeI+ AvrII | |
need to digest with Sal1 + age1 and Sal1 + BglII
Colony Screening
[edit]
Result intepretation/Summary
[edit]
Conclusion
[edit]
Cloning of 1017-pDNR-Dual-ATR-C-His
[edit]Purpose: Cloning of 1005-pBad24-Tat-sfGFP by PCR
Date: 2012-5-9--2012-
Notebook:0
Page:
Clone ID:1017
Experimenters: yingying
Results
[edit]cloning
[edit]Date:2012-5-9
| band 1 |
band 2 | |
| 1016,pDNR-Dual-ATR |
1016,pDNR-Dual-ATR | |
| enzymes |
Not1+Bgl11 |
Sal1+Bgl11 |
Colony Screening
[edit]Date:
Result intepretation/Summary
- <br>
Conclusion
[edit]<br>
<br>
<br>