1020-TurboRFP-TA vector
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Cloning of 1020-TurboRFP-TA vector
[edit]Purpose: Cloning of 1020-TurboRFP-TA vector
Date: 2012-3-5---3-7
Notebook:00
Page:
Clone ID:1020
Experimenters: yingying
Results
[edit]cloning
[edit]Date:2012-2-17
template:TurboRFP-1 to TurboRFP-20 mixture
primer F:TurboRFP-1
primer R:TurboRFP-20
TA ligate method
Colony Screening
[edit]Date: 2012-3-7
Make miniprep and digest with Xho1 + Xba1
clone 2,3,4,5,6,7,8,9 positive
Date: 2012-3-22
Make miniprep and digest with Xho1 /Xba1 /EcoR1
Result intepretation/Summary
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Conclusion
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