1021-pET8XHis-T5Exo
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Cloning of1021-pET8XHis-T5Exo
[edit]Purpose: Cloning of 1021-pET8XHis-T5Exo
Date: 2012-3-21--2012
Notebook:0
Page:
Clone ID:1021
Experimenters: yingying
Results
[edit]cloning
[edit]Date:2012-3-21
T5Exo-1 to T5Exo-22 gene synthesis
| band 5 |
band 6 | |
| PCR product |
pET8XHis 286 | |
| enzymes |
Nco1+ Xho1 | |
Colony Screening
[edit]Date: 2012-3-26
Make miniprep and digest with Nco1+ Xho1
clone 11,12,13,14,15 may be right
c
Result intepretation/Summary
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Conclusion
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