1032-pET6XHis-sso7d-wt-Taq

Cloning of pET6XHis-sso7d-wt-Taq (1032)
Purpose: to clone pET6XHis-sso7d-wt-Taq
Date: 2012-3-26 to 2012-3-30
Notebook: Xiaojing Wang
Page: 004206
Clone ID: 1032
Experimenters: Xiaojing Wang
Results

The bands show PCR product of 1032,

sso7d-Nde1-F
aca tac CAT ATG gGC AGC AGC CACCATCATCATCATCAT acg gtc aaa ttc aag tac aaag

sso7d-EcoR1-R

(G AAA AAA GGC GGT GGT GGC GAA TTC agagta)
tactctGAATTCGCCACCACCGCCTTTTTTC

Digest result:

digest PCR product and vector with Nde1+EcoR1, run gel

the left bands show 1032 insert, the right bands show 512 vector

gel purify, ligate 2 hours, transform Top10,K+

Colony Screening

1032-1,-2,-3 may be correct.

Select 1032-3 to sequence.