1033-pGST-DNase1

Cloning of pGST-DNase1 (1033)
Purpose: to clone pGST-DNase1
Date: 2012-3-21 to 2012-3-23
Notebook: Xiaojing Wang
Page: 004203
Clone ID: 1033
Experimenters: Xiaojing Wang
Results

digest pGST 455 vector with EcoR1 + Xho1, digest pGST-DNase1 pET28-DNase1 202 with EcoR1 + Xho1-- insert

Ligate, transform top10

Select 4 clones to mini-prep
1033 plasmids are digested by EcoR1 +Xho1

Clone 1033-1,-2,-3,-4 may be correct.